The lab states fixation will lead to the dyes to leak from the cells. I feel the assumption is always that fixation may harm cell membranes. I did look for a paper which describes fixation applying methanol or acetic acid, after staining using a DCFDA derivative. DCFDA could be the https://2-fdckhcl82580.blog2learn.com/76176064/the-definitive-guide-to-2-fdck-hcl